Plasmid_Backbone

Part:BBa_K2862020:Design

Designed by: Joshua Lawrence   Group: iGEM18_Imperial_College   (2018-10-09)


pSB1C3 for Multiple Assembly Standards (3A, BASIC and Golden Gate)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 75
    Illegal suffix found at 1068
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 75
    Illegal NheI site found at 121
    Illegal NheI site found at 144
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
    Illegal SpeI site found at 1069
    Illegal PstI site found at 1083
    Illegal NotI site found at 81
    Illegal NotI site found at 1076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 75
    Illegal BamHI site found at 873
    Illegal XhoI site found at 2100
    Illegal XhoI site found at 2992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 75
    Illegal suffix found at 1069
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 75
    Plasmid lacks a suffix.
    Illegal XbaI site found at 90
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
    Illegal SpeI site found at 1069
    Illegal PstI site found at 1083
    Illegal AgeI site found at 734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1054
    Illegal BsaI.rc site found at 104


Design Notes

pSB1C3_BASIC (iGEM vector with BASIC sites)



Source

pSB1C3_BASIC (iGEM vector with BASIC sites)


References